Practice Advisory: Vaginal Seeding

The American College of Ob/Gyn 

November 8, 2016

This is an area of evolving care and practice. Fellows should check periodically for revisions and updates. ACOG will communicate important changes and updates to these guidelines.

Vaginal seeding refers to a practice of using gauze or a swab to “transfer” vaginal fluids to an infant born via cesarean delivery. The theory behind this practice is that vaginal seeding, by exposing the infant to vaginal microbiota, would ameliorate the increased risk of autoimmune diseases, asthma, and allergic diseases seen in children not exposed to vaginal microbiota because they were delivered by cesarean in women who did not experience labor. The reduced risk of autoimmune disease, asthma, and allergic diseases observed in infants born vaginally is presumed to be associated with their intrapartum exposure to maternal vaginal microbiota (1, 2, 3, 4). It has been reported that infants born via cesarean delivery acquire different microbiota than vaginally delivered infants (5). In a study of 18 infants, of which 11 were born via cesarean delivery, swabbing the cesarean-born infant's mouth, face, and then the rest of the body was performed with a sterile gauze pad which had been placed in the mother’s vagina for one hour prior to delivery (5).  In this study, the vaginally delivered infants and those that underwent cesarean delivery with subsequent swabbing demonstrated a vaginal microbiome-like signature that was not present in the cesarean-delivered infants not exposed to the vaginal swabs (5).

While the theory of vaginal seeding may at first seem biologically plausible, it is critical to recognize that data regarding benefits and harms are lacking. It is also important to recognize that the transfer of some pathogens, which may be asymptomatic in the mother, could result in severe adverse consequences for infants, including group B streptococcus, herpes simplex virus, Chlamydia trachomatis, and Neisseria gonorrhea (6). Currently, there is only one clinical trial in the United States underway to investigate vaginal seeding (7), but the main outcome is neonatal microbiota and not clinical outcomes.

With the increasing attention given to this concept in public media, providers are being faced with incorporating vaginal seeding into clinical practices (6). At this time, based on the lack of data about the safety and harms, and the potential for harm from transfer of pathogens associated with adverse neonatal outcomes, we recommend against the practice of vaginal seeding until better data are available establishing the safety and benefits of this practice.


  1. Neu J, Rushing J. Cesarean versus vaginal delivery: long-term infant outcomes and the hygiene hypothesis. Clin Perinatol 2011;38:321-31.
  2. Mueller NT, Bakacs E, Combellick J, Grigoryan Z, Dominguez-Bello MG. The infant microbiome development: mom matters. Trends Mol Med 2015;21:109-17.
  3. Sevelsted A, Stokholm J, Bonnelykke K, Bisgaard H. Cesarean section and chronic immune disorders. Pediatrics 2015;135:e92-8.
  4. Arrieta MC, Stiemsma LT, Dimitriu PA, Thorson L, Russell S, Yurist-Doutsch S, et al. Early infancy microbial and metabolic alterations affect risk of childhood asthma. Sci Transl Med 2015;7:307ra152.
  5. Dominguez-Bello MG, De Jesus-Laboy KM, Shen N, Cox LM, Amir A, Gonzalez A, et al. Partial restoration of the microbiota of cesarean-born infants via vaginal microbial transfer. Nat Med 2016;22:250-3.
  6. Cunnington AJ, Sim K, Deierl A, Kroll JS, Brannigan E, Darby J. "Vaginal seeding" of infants born by caesarean section. BMJ 2016;352:i227.
  7. New York University School of Medicine. Potential Restoration of the Infant Microbiome (PRIME). In: Bethesda (MD): National Library of Medicine; 2015. Available at: Retrieved October 28, 2016.

This Practice Advisory was developed by the American College of Obstetricians and Gynecologists in collaboration with Christopher M. Zahn, MD.

A Practice Advisory is issued when information on an emergent clinical issue (e.g. clinical study, scientific report, draft regulation) is released that requires an immediate or rapid response, particularly if it is anticipated that it will generate a multitude of inquiries. A Practice Advisory is a brief, focused statement issued within 24-48 hours of the release of this evolving information and constitutes ACOG clinical guidance. A Practice Advisory is issued only on-line for Fellows but may also be used by patients and the media. Practice Advisories are reviewed periodically for reaffirmation, revision, withdrawal or incorporation into other ACOG guidelines.

This document reflects emerging clinical and scientific advances as of the date issued and is subject to change. The information should not be construed as dictating an exclusive course of treatment or procedure to be followed.           

The American College of Obstetricians and Gynecologists (The College), a 501(c)(3) organization, is the nation's leading group of physicians providing health care for women. As a private, voluntary, nonprofit membership organization of more than 57,000 members, The College strongly advocates for quality health care for women, maintains the highest standards of clinical practice and continuing education of its members, promotes patient education, and increases awareness among its members and the public of the changing issues facing women’s health care. The American Congress of Obstetricians and Gynecologists (ACOG), a 501(c)(6) organization, is its companion organization.

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